Modular epistasis and the compensatory evolution of gene deletion mutants.

ABSTRACT: Screens for epistatic interactions have long been used to characterize functional relationships corresponding to protein complexes, metabolic pathways, and other functional modules. Although epistasis between adaptive mutations is also common in laboratory evolution experiments, the functional basis for these interactions is less well characterized. Here, we quantify the extent to which gene function (as determined by a genome-wide screen for epistasis among deletion mutants) influences the rate and genetic basis of compensatory adaptation in a set of 37 gene deletion mutants nested within 16 functional modules. We find that functional module has predictive power: mutants with deletions in the same module tend to adapt more similarly, on average, than those with deletions in different modu

SUBMITTER: Rojas Echenique JI 

PROVIDER: S-EPMC6395002 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature