Cyclical adaptation of measles virus quasispecies to epithelial and lymphocytic cells: To V, or not to V.

ABSTRACT: Measles virus (MeV) is dual-tropic: it replicates first in lymphatic tissues and then in epithelial cells. This switch in tropism raises the question of whether, and how, intra-host evolution occurs. Towards addressing this question, we adapted MeV either to lymphocytic (Granta-519) or epithelial (H358) cells. We also passaged it consecutively in both human cell lines. Since passaged MeV had different replication kinetics, we sought to investigate the underlying genetic mechanisms of growth differences by performing deep-sequencing analyses. Lymphocytic adaptation reproducibly resulted in accumulation of variants mapping within an 11-nucleotide sequence located in the middle of the phosphoprotein (P) gene. This sequence mediates polymerase slippage and addition of a pseudo-templated guanos

SUBMITTER: Donohue RC 

PROVIDER: S-EPMC6395005 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature