Electrical stimulation increases hypertrophy and metabolic flux in tissue-engineered human skeletal muscle.

ABSTRACT: In vitro models of contractile human skeletal muscle hold promise for use in disease modeling and drug development, but exhibit immature properties compared to native adult muscle. To address this limitation, 3D tissue-engineered human muscles (myobundles) were electrically stimulated using intermittent stimulation regimes at 1 Hz and 10 Hz. Dystrophin in myotubes exhibited mature membrane localization suggesting a relatively advanced starting developmental maturation. One-week stimulation significantly increased myobundle size, sarcomeric protein abundance, calcium transient amplitude (∼2-fold), and tetanic force (∼3-fold) resulting in the highest specific force generation (19.3mN/mm²) reported for engineered human muscles to date. Compared to 1 Hz electrical stimulation, the 1

SUBMITTER: Khodabukus A 

PROVIDER: S-EPMC6395553 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature