Viscoelastic Separation and Concentration of Fungi from Blood for Highly Sensitive Molecular Diagnostics.

ABSTRACT: Isolation and concentration of fungi in the blood improves sensitivity of the polymerase chain reaction (PCR) method to detect fungi in blood. This study demonstrates a sheathless, continuous separation and concentration method of candida cells using a viscoelastic fluid that enables rapid detection of rare candida cells by PCR analysis. To validate device performance using a viscoelastic fluid, flow characteristics of 2 μm particles were estimated at different flow rates. Additionally, a mixture of 2 μm and 13 μm particles was successfully separated based on size difference at 100 μl/min. Candida cells were successfully separated from the white blood cells (WBCs) with a separation efficiency of 99.1% and concentrated approximately 9.9-fold at the center outlet compared to the initial conc

SUBMITTER: Nam J 

PROVIDER: S-EPMC6395622 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature