DeMix: Decoding Deuterated Distributions from Heterogeneous Protein States via HDX-MS.

ABSTRACT: Characterization of protein structural changes in response to protein modifications, ligand or chemical binding, or protein-protein interactions is essential for understanding protein function and its regulation. Amide hydrogen/deuterium exchange (HDX) coupled with mass spectrometry (MS) is one of the most favorable tools for characterizing the protein dynamics and changes of protein conformation. However, currently the analysis of HDX-MS data is not up to its full power as it still requires manual validation by mass spectrometry experts. Especially, with the advent of high throughput technologies, the data size grows everyday and an automated tool is essential for the analysis. Here, we introduce a fully automated software, referred to as 'deMix', for the HDX-MS data analysis. deMix deals

SUBMITTER: Na S 

PROVIDER: S-EPMC6395696 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature