Atg7-dependent canonical autophagy regulates the degradation of aquaporin 2 in prolonged hypokalemia.

ABSTRACT: Prolonged hypokalemia induces a decrease of urinary concentrating ability via down-regulation of aquaporin 2 (AQP2); however, the precise mechanisms remain unknown. To investigate the role of autophagy in the degradation of AQP2, we generated the principal cell-specific Atg7 deletion (Atg7^Δpc) mice. In hypokalemic Atg7-floxed (Atg7^f/f) mice, huge irregular shaped LC3-positive autophagic vacuoles accumulated mainly in inner medullary collecting duct (IMCD) cells. Total- and pS261-AQP2 were redistributed from apical and subapical domains into these vacuoles, which were not co-localized with RAB9. However, in the IMCD cells of hypokalemic Atg7^Δpc mice, these canonical autophagic vacuoles were markedly reduced, whereas numerous small regular shaped LC3-negative

SUBMITTER: Kim WY 

PROVIDER: S-EPMC6395725 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature