Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia.

ABSTRACT: Culture-independent methods have contributed substantially to our understanding of global microbial diversity. Recently developed algorithms to construct whole genomes from environmental samples have further refined, corrected and revolutionized understanding of the tree of life. Here, we assembled draft metagenome-assembled genomes (MAGs) from environmental DNA extracted from two hot springs within an active volcanic ecosystem on the Kamchatka peninsula, Russia. This hydrothermal system has been intensively studied previously with regard to geochemistry, chemoautotrophy, microbial isolation, and microbial diversity. We assembled genomes of bacteria and archaea using DNA that had previously been characterized via 16S rRNA gene clone libraries. We recovered 36 MAGs, 29 of medium to high qua

SUBMITTER: Wilkins LGE 

PROVIDER: S-EPMC6395817 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature