Clofazimine Exposure In Vitro Selects Efflux Pump Mutants and Bedaquiline Resistance.

ABSTRACT: Six in vitro clofazimine-resistant spontaneous mutants obtained from a wild-type or pyrazinamide-resistant ATCC reference strain were selected to evaluate bedaquiline cross-resistance. The reverse was conducted for bedaquiline mutants. All clofazimine mutants harboring an rv0678 mutation displayed phenotypic cross-resistance. We observed the same for rv0678 bedaquiline mutants; however, atpE bedaquiline mutants showed no phenotypic cross-resistance. This confirms that upfront clofazimine usage may impact subsequent bedaquiline use due to a shared efflux resistance pathway.

SUBMITTER: Ismail N 

PROVIDER: S-EPMC6395909 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature