Detection of Echinocandin-Resistant Candida glabrata in Blood Cultures Spiked with Different Percentages of FKS2 Mutants.

ABSTRACT: Infections caused by the coexistence of Candida glabrata echinocandin-resistant and echinocandin-susceptible cells may be possible, and the detection of FKS mutants when the proportions of FKS mutants are underrepresented poses a problem. We assessed the role of EUCAST and methods directly performed on positive blood cultures-Etest (ETDIR) and anidulafungin-containing agar plate assays-for detecting resistance in C. glabrata isolates containing different amounts of echinocandin-susceptible and -resistant Candida glabrata isolates. We studied 10 pairs of C. glabrata isolates involving parental echinocandin-susceptible isolates and isogenic echinocandin-resistant FKS mutant isolates. Three inocula per pair (1 ×  103 to 5  ×  103, 1 ×  102 to 5  ×  102, and 10 to 50 CFU/ml) spanning suspensio

SUBMITTER: Bordallo-Cardona MA 

PROVIDER: S-EPMC6395934 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature