Effects of the dual orexin receptor antagonist DORA-22 on sleep in 5XFAD mice.

ABSTRACT:

Introduction

Sleep disruption is a characteristic of Alzheimer's disease (AD) that may exacerbate disease progression. This study tested whether a dual orexin receptor antagonist (DORA) would enhance sleep and attenuate neuropathology, neuroinflammation, and cognitive deficits in an AD-relevant mouse model, 5XFAD.

Methods

Wild-type (C57Bl6/SJL) and 5XFAD mice received chronic treatment with vehicle or DORA-22. Piezoelectric recordings monitored sleep and spatial memory was assessed via spontaneous Y-maze alternations. Aβ plaques, Aβ levels, and neuroinflammatory markers were measured by immunohistochemistry, enzyme-linked immunosorbent assay, and real-time polymerase chain reaction, respectively.

Results

In 5XFAD mice, DORA-22 significantly increased light-phase sleep

SUBMITTER: Duncan MJ 

PROVIDER: S-EPMC6396100 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature