Microheterogeneity of Topoisomerase IA/IB and Their DNA-Bound States.

ABSTRACT: Topoisomerases are important complex enzymes that modulate DNA topology to maintain chromosome superstructure and integrity. These enzymes are involved in many cellular processes that resolve specific DNA superstructures and intermediates. The low abundance combined with the biological heterogeneity of relevant intermediates of topoisomerases makes their structural information not readily accessible using traditional structural biology tools (e.g., NMR and X-ray crystallography). In the present work, a second-generation trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) was used to study Escherichia coli topoisomerase IA (EcTopIA) and variola virus topoisomerase IB (vTopIB) as well as their complexes with a single-stranded DNA and a stem-loop DNA under native condi

SUBMITTER: Jeanne Dit Fouque K 

PROVIDER: S-EPMC6396120 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature