A topological view of human CD34⁺ cell state trajectories from integrated single-cell output and proteomic data.

ABSTRACT: Recent advances in single-cell molecular analytical methods and clonal growth assays are enabling more refined models of human hematopoietic lineage restriction processes to be conceptualized. Here, we report the results of integrating single-cell proteome measurements with clonally determined lymphoid, neutrophilic/monocytic, and/or erythroid progeny outputs from >1000 index-sorted CD34⁺ human cord blood cells in short-term cultures with and without stromal cells. Surface phenotypes of functionally examined cells were individually mapped onto a molecular landscape of the entire CD34⁺ compartment constructed from single-cell mass cytometric measurements of 14 cell surface markers, 20 signaling/cell cycle proteins, and 6 transcription factors in ∼300 000 cells. This an

SUBMITTER: Knapp DJHF 

PROVIDER: S-EPMC6396178 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature