Large-scale discovery of mouse transgenic integration sites reveals frequent structural variation and insertional mutagenesis.

ABSTRACT: Transgenesis has been a mainstay of mouse genetics for over 30 yr, providing numerous models of human disease and critical genetic tools in widespread use today. Generated through the random integration of DNA fragments into the host genome, transgenesis can lead to insertional mutagenesis if a coding gene or an essential element is disrupted, and there is evidence that larger scale structural variation can accompany the integration. The insertion sites of only a tiny fraction of the thousands of transgenic lines in existence have been discovered and reported, due in part to limitations in the discovery tools. Targeted locus amplification (TLA) provides a robust and efficient means to identify both the insertion site and content of transgenes through deep sequencing of genomic loci linked

SUBMITTER: Goodwin LO 

PROVIDER: S-EPMC6396414 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature