Multiscale modeling of influenza A virus replication in cell cultures predicts infection dynamics for highly different infection conditions.

ABSTRACT: Influenza A viruses (IAV) are commonly used to infect animal cell cultures for research purposes and vaccine production. Their replication is influenced strongly by the multiplicity of infection (MOI), which ranges over several orders of magnitude depending on the respective application. So far, mathematical models of IAV replication have paid little attention to the impact of the MOI on infection dynamics and virus yields. To address this issue, we extended an existing model of IAV replication in adherent MDCK cells with kinetics that explicitly consider the time point of cell infection. This modification does not only enable the fitting of high MOI measurements, but also the successful prediction of viral release dynamics of low MOI experiments using the same set of parameters. Furthermo

SUBMITTER: Rudiger D 

PROVIDER: S-EPMC6396949 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature