High accuracy, fiducial marker-based image registration of correlative microscopy images.

ABSTRACT: Fluorescence microscopy (FM) and electron microscopy (EM) are complementary techniques. FM affords examination of large fields of view and identifying regions of interest but has a low resolution. EM exhibits excellent resolution over a limited field of view. The combination of these two techniques, correlative microscopy, received considerable interest in the past years and has proven its potential in biology and material science. Accurate correlation of FM and EM images is, however, challenging due to the differences in contrast mechanism, size of field of view and resolution. We report an accurate, fast and robust method to correlate FM and EM images using low densities of fiducial markers. Here, 120 nm diameter fiducial markers consisting of fluorescently labelled silica coated gold na

SUBMITTER: Mohammadian S 

PROVIDER: S-EPMC6397213 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature