Rapid measurement of hydrogen sulphide in human blood plasma using a microfluidic method.

ABSTRACT: Hydrogen sulfide (H₂S) is emerging as an important gasotransmitter in both physiological and pathological states. Rapid measurement of H₂S remains a challenge. We report a microfluidic method for rapid measurement of sulphide in blood plasma using Dansyl-Azide, a fluorescence (FL) based probe. We have measured known quantities of externally added (exogenous) H₂S to both buffer and human blood plasma. Surprisingly, a decrease in FL intensity with increase in exogenous sulphide concentration in plasma was observed which is attributed to the interaction between the proteins and sulphide present in plasma underpinning our observation. The effects of mixing and incubation time, pH, and dilution of plasma on the FL intensity is studied which revealed that the FL

SUBMITTER: Karunya R 

PROVIDER: S-EPMC6397262 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature