A frame-shifted gene, which rescued its function by non-natural start codons and its application in constructing synthetic gene circuits.

ABSTRACT:

Background

Frame-shifted genes results in non-functional peptides. Because of this complete loss of function, frame-shifted genes have never been used in constructing synthetic gene circuits.

Results

Here we report that the function of gene circuits is rescued by a frame-shifted gene, which functions by translating from a non-natural start codon. We report a single nucleotide deletion mutation that developed in the λ-repressor cI within a synthetic genetic NOT gate in Escherichia coli during growth and through this mutation, a non-functional synthetic gene circuit became functional. This mutation resulted in a frame-shifted cI, which showed effective functionality among genetic NOT-gates in Escherichia coli with high regulatory ranges (> 300) and Hill coefficie

SUBMITTER: Sarkar K 

PROVIDER: S-EPMC6397469 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature