Genome replication affects transcription factor binding mediating the cascade of herpes simplex virus transcription.

ABSTRACT: In herpes simplex virus type 1 (HSV-1) infection, the coupling of genome replication and transcription regulation has been known for many years; however, the underlying mechanism has not been elucidated. We performed a comprehensive transcriptomic assessment and factor-binding analysis for Pol II, TBP, TAF1, and Sp1 to assess the effect genome replication has on viral transcription initiation and elongation. The onset of genome replication resulted in the binding of TBP, TAF1, and Pol II to previously silent late promoters. The viral transcription factor, ICP4, was continuously needed in addition to DNA replication for activation of late gene transcription initiation. Furthermore, late promoters contain a motif that closely matches the consensus initiator element (Inr), which robustly boun

SUBMITTER: Dremel SE 

PROVIDER: S-EPMC6397523 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature