Cold sensing by Na_V1.8-positive and Na_V1.8-negative sensory neurons.

ABSTRACT: The ability to detect environmental cold serves as an important survival tool. The sodium channels Na_V1.8 and Na_V1.9, as well as the TRP channel Trpm8, have been shown to contribute to cold sensation in mice. Surprisingly, transcriptional profiling shows that Na_V1.8/Na_V1.9 and Trpm8 are expressed in nonoverlapping neuronal populations. Here we have used in vivo GCaMP3 imaging to identify cold-sensing populations of sensory neurons in live mice. We find that ∼80% of neurons responsive to cold down to 1 °C do not express Na_V1.8, and that the genetic deletion of Na_V1.8 does not affect the relative number, distribution, or maximal response of cold-sensitive neurons. Furthermore, the deletion of Na_V1.8 had no observabl

SUBMITTER: Luiz AP 

PROVIDER: S-EPMC6397562 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature