Epithelial-to-mesenchymal transition of A549 lung cancer cells exposed to electronic cigarettes.

ABSTRACT:

Objectives

Epithelial-to-mesenchymal transition (EMT) is the initial step enabling the metastasis of cancer cells, which often leads to death. Although smoking is a major risk factor for lung cancer, there is still widespread use of conventional cigarettes. Recently, the tobacco industry has been transformed by the introduction of electronic cigarettes (ECs), which have lower levels of carcinogens and may provide a safer alternative. Here, we investigate the ability of EC liquids and aerosols to induce an EMT in A549 lung cancer cells.

Materials and methods

Human adenocarcinoma alveolar basal epithelial cells (A549) were exposed to EC liquids and aerosols from a popular product for 3-8 days. Live cell imaging, EMT biomarker analysis, and machine learning/image processing alg

SUBMITTER: Zahedi A 

PROVIDER: S-EPMC6397801 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature