HIV-1 envelope proteins up-regulate N ⁶-methyladenosine levels of cellular RNA independently of viral replication.

ABSTRACT: N ⁶-methyladenosine (m⁶A) modification of HIV-1 RNA regulates viral replication and protein expression. The m⁶A modification is regulated by two groups of cellular proteins named writers and erasers that add or remove m⁶A, respectively. HIV-1 infection of CD4⁺ T-cells increases m⁶A levels of cellular mRNA, but the underlying mechanism is unknown. Here, we show that HIV-1 infection of CD4⁺ primary T-cells or Jurkat cells significantly increases m⁶A levels of cellular RNA independently of viral replication. Compared with HIV-1-infected CD4⁺ T-cells, similar m⁶A up-regulation was detected in total RNA from HIV-1-infected cells treated with a reverse-transcriptase inhibitor or w

SUBMITTER: Tirumuru N 

PROVIDER: S-EPMC6398121 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature