Milk biosynthesis requires the Golgi cation exchanger TMEM165.

ABSTRACT: Milk is a hallmark of mammals that is critical for normal growth and development of offspring. During biosynthesis of lactose in the Golgi complex, H⁺ is produced as a by-product, and there is no known mechanism for maintaining luminal pH within the physiological range. Here, using conditional, tissue-specific knockout mice, immunostaining, and biochemical assays, we test whether the putative H⁺/Ca²⁺/Mn²⁺ exchanger known as TMEM165 (transmembrane protein 165) participates in normal milk production. We find TMEM165 is crucial in the lactating mammary gland for normal biosynthesis of lactose and for normal growth rates of nursing pups. The milk of TMEM165-deficient mice contained elevated concentrations of fat, protein, iron, and zinc, which are li

SUBMITTER: Snyder NA 

PROVIDER: S-EPMC6398142 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature