Effect of the MotA(M206I) Mutation on Torque Generation and Stator Assembly in the Salmonella H+-Driven Flagellar Motor.

ABSTRACT: The bacterial flagellar motor is composed of a rotor and a dozen stators and converts the ion flux through the stator into torque. Each stator unit alternates in its attachment to and detachment from the rotor even during rotation. In some species, stator assembly depends on the input energy, but it remains unclear how an electrochemical potential across the membrane (e.g., proton motive force [PMF]) or ion flux is involved in stator assembly dynamics. Here, we focused on pH dependence of a slow motile MotA(M206I) mutant of Salmonella The MotA(M206I) motor produces torque comparable to that of the wild-type motor near stall, but its rotation rate is considerably decreased as the external load is reduced. Rotation assays of flagella labeled with 1-μm beads showed that the rotation rate of t

SUBMITTER: Suzuki Y 

PROVIDER: S-EPMC6398272 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature