Use of Next Generation Sequencing to study two cowpox virus outbreaks.

ABSTRACT:

Background

Between 2008 and 2011 about 40 cases of human cowpox were reported from Germany and France. Infections had been acquired via close contact to infected, young pet rats. An identical and unique sequence of the hemagglutinin gene was found in various cowpox virus (CPXV) isolates pointing to a common source of infection. In a second CPXV outbreak in cats in a small animal clinic in Germany in 2015, four out of five hospitalized cats showed identical hemagglutinin sequences and thus, a hospital-acquired transmission had been assumed. Next-Generation Sequencing was performed in order to re-investigate the outbreaks, as epidemiological data could not confirm all cases.

Methods

Homogenates of lesion material from rats, cats and humans were cultivated in cell culture. The

SUBMITTER: Antwerpen MH 

PROVIDER: S-EPMC6398431 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature