ProLIF - quantitative integrin protein-protein interactions and synergistic membrane effects on proteoliposomes.

ABSTRACT: Integrin transmembrane receptors control a wide range of biological interactions by triggering the assembly of large multiprotein complexes at their cytoplasmic interface. Diverse methods have been used to investigate interactions between integrins and intracellular proteins, and predominantly include peptide-based pulldowns and biochemical immuno-isolations from detergent-solubilised cell lysates. However, quantitative methods to probe integrin-protein interactions in a more biologically relevant context where the integrin is embedded within a lipid bilayer have been lacking. Here, we describe 'protein-liposome interactions by flow cytometry' (denoted ProLIF), a technique to reconstitute recombinant integrin transmembrane domains (TMDs) and cytoplasmic tail (CT) fragments in liposomes as

SUBMITTER: De Franceschi N 

PROVIDER: S-EPMC6398480 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature