Microtubule dynamics regulation reconstituted in budding yeast lysates.

ABSTRACT: Microtubules (MTs) are important for cellular structure, transport of cargoes and segregation of chromosomes and organelles during mitosis. The stochastic growth and shrinkage of MTs, known as dynamic instability, is necessary for these functions. Previous studies to determine how individual MT-associated proteins (MAPs) affect MT dynamics have been performed either through in vivo studies, which provide limited opportunity for observation of individual MTs or manipulation of conditions, or in vitro studies, which focus either on purified proteins, and therefore lack cellular complexity, or on cell extracts made from genetically intractable organisms. In order to investigate the ensemble activities of all MAPs on MT dynamics using lysates made from a genetically tractable org

SUBMITTER: Bergman ZJ 

PROVIDER: S-EPMC6398484 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature