Utility of a portable desiccant system for preservation of fecal samples for downstream 16S rRNA amplicon sequencing.

ABSTRACT: While recent advances in culture-independent sequencing approaches have revitalized the field of microbiology, rapid collection and preservation of microbial DNA in samples like feces is critical to avoid degradation of target DNA via nuclease activity and proliferation of aerotolerant microbes. Common laboratory practices to ameliorate such changes rely on prompt freezing of samples or dispersion in nuclease-inhibiting reagents. As many of the microbial enzymes associated with nuclease activity and bacterial proliferation are hydrolases, prompt desiccation of samples offers an attractive alternative to freezing and liquid reagents for field collection of samples in remote areas. Herein, we evaluated the utility of a portable desiccant chamber with a rechargeable cartridge, for preservatio

SUBMITTER: Johnson PJ 

PROVIDER: S-EPMC6398931 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature