Calcium-Phosphate Crystals Promote RANKL Expression via the Downregulation of DUSP1.

ABSTRACT: Osteoarthritis (OA) is a naturally occurring, irreversible disorder and a major health burden. The disease is multifactorial, involving both physiological and mechanical processes, but calcium crystals have been associated intimately with its pathogenesis. This study tested the hypothesis that these crystals have a detrimental effect on the differentiation of osteoclasts and bone homeostasis. This study employed an osteoblast-osteoclast coculture system that resembles in vivo osteoblast-dependent osteoclast differentiation along with Ca²⁺-phosphate-coated culture dishes. The calcium-containing crystals upregulated the expression of RANKL and increased the differentiation of osteoclasts significantly as a result. On the other hand, osteoblast differentiation was unaffected

SUBMITTER: Choi Y 

PROVIDER: S-EPMC6399012 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature