The Impact of Varying Cooling and Thawing Rates on the Quality of Cryopreserved Human Peripheral Blood T Cells.

ABSTRACT: For the clinical delivery of immunotherapies it is anticipated that cells will be cryopreserved and shipped to the patient where they will be thawed and administered. An established view in cellular cryopreservation is that following freezing, cells must be warmed rapidly (≤5 minutes) in order to maintain high viability. In this study we examine the interaction between the rate of cooling and rate of warming on the viability, and function of T cells formulated in a conventional DMSO based cryoprotectant and processed in conventional cryovials. The data obtained show that provided the cooling rate is -1 °C min^-1 or slower, there is effectively no impact of warming rate on viable cell number within the range of warming rates examined (1.6 °C min^-1 to 113 °C min^-1</

SUBMITTER: Baboo J 

PROVIDER: S-EPMC6399228 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature