GM-CSF Promotes the Expansion and Differentiation of Cord Blood Myeloid-Derived Suppressor Cells, Which Attenuate Xenogeneic Graft-vs.-Host Disease.

ABSTRACT: Myeloid-derived suppressor cells (MDSCs) are increased in tumor patients. Studies have shown generation of MDSCs from human peripheral blood mononuclear cells (PBMCs) by various cytokine combinations. However, large scale expansion of human MDSCs has not been demonstrated or applied in clinic settings. We investigated which cytokine combinations among GM-CSF/SCF, G-CSF/SCF, or M-CSF/SCF efficiently expand and differentiate human MDSCs following culture CD34⁺ cells of umbilical cord blood (CB). GM-CSF/SCF showed the greatest expansion of MDSCs. Up to 10⁸ MDSCs (HLA-DR^lowCD11b⁺CD33⁺) could be produced from 1 unit of CB following 6 weeks of continuous culture. MDSCs produced from culture of CD34⁺ cells with GM-CSF/SCF for 6 wee

SUBMITTER: Park MY 

PROVIDER: S-EPMC6399310 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature