Evaluation of a Dual Isolation Width Acquisition Method for Isobaric Labeling Ratio Decompression.

ABSTRACT: Isobaric labeling is a highly precise approach for protein quantification. However, due to the isolation interference problem, isobaric tagging suffers from ratio underestimation at the MS2 level. The use of narrow isolation widths is a rational approach to alleviate the interference problem; however, this approach compromises proteome coverage. We reasoned that although a very narrow isolation window will result in loss of peptide fragment ions, the reporter ion signals will be retained for a significant portion of the spectra. On the basis of this assumption, we have designed a dual isolation width acquisition (DIWA) method, in which each precursor is first fragmented with HCD using a standard isolation width for peptide identification and preliminary quantification, followed by a second

SUBMITTER: Roumeliotis TI 

PROVIDER: S-EPMC6399672 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature