Regulation of platelet-activating factor-induced interleukin-8 expression by protein tyrosine phosphatase 1B.

ABSTRACT:

Background

Platelet-activating factor (PAF) is a potent lipid mediator whose involvement in the onset and progression of atherosclerosis is mediated by, among others, the modulation of cytokine expression patterns. The presence of multiple potential protein-tyrosine phosphatase (PTP) 1B substrates in PAF receptor signaling pathways brought us to investigate its involvement in PAF-induced cytokine expression in monocyte-derived dendritic cells (Mo-DCs) and to study the pathways involved in this modulation.

Methods

We used in-vitro-matured human dendritic cells and the HEK-293 cell line in our studies. PTP1B inhibition was though siRNAs and a selective inhibitor. Cytokine expression was studied with RT-PCR, luciferase assays and ELISA. Phosphorylation status of kinases and tra

SUBMITTER: Hamel-Cote G 

PROVIDER: S-EPMC6399872 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature