Cytology and molecular study for GSTP1 effect on asthma Iraqi patients.

ABSTRACT:

Background

GST belongs to a super family of phase II detoxification enzyme and it plays an important role in preventing the damage that may occur due to reactive water-soluble compounds generated by the association of reactive intermediates with glutathione.

Method

In the present study, we analyzed the frequencies of GSTP1 polymorphism among the Iraqi population using PCR-RFLP technique. Fifty samples from bronchial asthma patients and fifty samples from control cases were subjected to conventional PCR and Restriction Fragment Length Polymorphism (RFLP) to detect GSTP1 genotype and measured different parameters together such as IgE, eosinophilic count, WBC, and so forth. Some of the cases were made to undergo sequence analysis and enrolled in NCBI GenBank with accession numb

SUBMITTER: Hamzah IH 

PROVIDER: S-EPMC6399971 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature