Engineering a Microbial Consortium Based Whole-Cell System for Efficient Production of Glutarate From L-Lysine.

ABSTRACT: Glutarate is an important C5 platform chemical produced during the catabolism of L-lysine through 5-aminovalerate (5-AMV) pathway. Here, we first established a whole-cell biocatalysis system for the glutarate production from L-lysine with the engineered Escherichia coli (E. coli) that co-expressed DavAB and GabDT. However, the accumulation of intermediate 5-AMV was identified as one important factor limiting glutarate production. Meanwhile, the negative interaction of co-expressing DavAB and GabDT in a single cell was also confirmed. Here, we solved these problems through engineering a microbial consortium composed of two engineered E. coli strains, BL21-22AB and BL21-YDT, as the whole-cell biocatalysts, each of which contains a part of the

SUBMITTER: Wang X 

PROVIDER: S-EPMC6400078 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature