Calixarene-mediated assembly of a small antifungal protein.

ABSTRACT: Synthetic macrocycles such as calixarenes and cucurbiturils are increasingly applied as mediators of protein assembly and crystallization. The macrocycle can facilitate assembly by providing a surface on which two or more proteins bind simultaneously. This work explores the capacity of the sulfonato-calix[n]arene (sclx _n ) series to effect crystallization of PAF, a small, cationic antifungal protein. Co-crystallization with sclx₄, sclx₆ or sclx₈ led to high-resolution crystal structures. In the absence of sclx _n , diffraction-quality crystals of PAF were not obtained. Interestingly, all three sclx _n were bound to a similar patch on PAF. The largest and most flexible variant, sclx₈, yiel

SUBMITTER: Alex JM 

PROVIDER: S-EPMC6400181 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature