A three-domain copper-nitrite reductase with a unique sensing loop.

ABSTRACT: Dissimilatory nitrite reductases are key enzymes in the denitrification pathway, reducing nitrite and leading to the production of gaseous products (NO, N₂O and N₂). The reaction is catalysed either by a Cu-containing nitrite reductase (NirK) or by a cytochrome cd ₁ nitrite reductase (NirS), as the simultaneous presence of the two enzymes has never been detected in the same microorganism. The thermophilic bacterium Thermus scotoductus SA-01 is an exception to this rule, harbouring both genes within a denitrification cluster, which encodes for an atypical NirK. The crystal structure of TsNirK has been determined at 1.63 Å resolution. TsNirK is a homotrimer with subunits of 451 residues that contain three copper atoms each. The N-t

SUBMITTER: Opperman DJ 

PROVIDER: S-EPMC6400189 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature