Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography.

ABSTRACT: The ability to determine high-quality, artefact-free structures is a challenge in micro-crystallography, and the rapid onset of radiation damage and requirement for a high-brilliance X-ray beam mean that a multi-crystal approach is essential. However, the combination of crystal-to-crystal variation and X-ray-induced changes can make the formation of a final complete data set challenging; this is particularly true in the case of metalloproteins, where X-ray-induced changes occur rapidly and at the active site. An approach is described that allows the resolution, separation and structure determination of crystal polymorphs, and the tracking of radiation damage in microcrystals. Within the microcrystal population of copper nitrite reductase, two polymorphs with different unit-cell sizes were

SUBMITTER: Ebrahim A 

PROVIDER: S-EPMC6400251 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature