Models to determine the kinetic mechanisms of ion-coupled transporters.

ABSTRACT: With high-resolution structures available for many ion-coupled (secondary active) transporters, a major challenge for the field is to determine how coupling is accomplished. Knowledge of the kinetic mechanism of the transport reaction, which defines the binding order of substrate and co-ions, together with the sequence with which all relevant states are visited by the transporter, will help to reveal this coupling mechanism. Here, we derived general mathematical models that can be used to analyze data from steady-state transport measurements and show how kinetic mechanisms can be derived. The models describe how the apparent maximal rate of substrate transport depends on the co-ion concentration, and vice versa, in different mechanisms. Similarly, they describe how the apparent affinity fo

SUBMITTER: Lolkema JS 

PROVIDER: S-EPMC6400521 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature