The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells.

ABSTRACT: H₂O₂ is a reactive oxygen species (ROS), which can diffuse away from its site of generation and may act as a cell-to-cell signaling factor. The mechanisms responsible for the generation of H₂O₂ in human ovarian follicles and possible signaling role(s) of H₂O₂ are not well known. We identified a source of H₂O₂, the enzyme NADPH oxidase (NOX) 4, in isolated differentiated, in-vitro fertilisation-derived human granulosa-lutein cells (GCs), in proliferating human granulosa tumour cells (KGN), as well as in situ in cells of growing ovarian follicles. H₂O₂ was readily detected in the supernatant of cultured GCs and KGN cells. H₂O₂ levels were significantly lowered b

SUBMITTER: Buck T 

PROVIDER: S-EPMC6400953 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature