Platelet lysate outperforms FCS and human serum for co-culture of primary human macrophages and hMSCs.

ABSTRACT: In vitro co-cultures of different primary human cell types are pivotal for the testing and evaluation of biomaterials under conditions that are closer to the human in vivo situation. Especially co-cultures of macrophages and mesenchymal stem cells (MSCs) are of interest, as they are both present and involved in tissue regeneration and inflammatory reactions and play crucial roles in the immediate inflammatory reactions and the onset of regenerative processes, thus reflecting the decisive early phase of biomaterial contact with the host. A co-culture system of these cell types might thus allow for the assessment of the biocompatibility of biomaterials. The establishment of such a co-culture is challenging due to the different in vitro cell culture conditions. For human macrophages, medium i

SUBMITTER: Tylek T 

PROVIDER: S-EPMC6401182 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature