Automated quantification of microvascular perfusion.

ABSTRACT:

Objective

Changes in microvascular perfusion have been reported in many diseases, yet the functional significance of altered perfusion is often difficult to determine. This is partly because commonly used techniques for perfusion measurement often rely on either indirect or by-hand approaches.

Methods

We developed and validated a fully automated software technique to measure microvascular perfusion in videos acquired by fluorescence microscopy in the mouse gastrocnemius. Acute perfusion responses were recorded following intravenous injections with phenylephrine, SNP, or saline.

Results

Software-measured capillary flow velocity closely correlated with by-hand measured flow velocity (R²  = 0.91, P < 0.0001). Software estimates of capillary hematocrit also gen

SUBMITTER: McClatchey PM 

PROVIDER: S-EPMC6401325 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature