Detection of pathogens in Dermacentor reticulatus in northwestern Europe: evaluation of a high-throughput array.

ABSTRACT:

Background

The geographic distribution of Dermacentor reticulatus is expanding in Europe. Surveillance of this tick species and its pathogens is desirable, as it transmits pathogens of public and veterinary importance. A high-throughput real-time PCR-based array was used to screen 1.741 D. reticulatus ticks from Belgium, Germany, The Netherlands, and Great Britain for the presence of 28 tick-borne bacteria and twelve protozoan parasites. The presence of pathogen DNA was confirmed by conventional PCR followed by sequencing.

Results

The array detected the presence of DNA from Borrelia spp. (7%), B. afzelii (0.1%), B. garinii (0.1%), B. spielmanii (0.1%), B. miyamotoi (0.2%), Anaplasma marginale (0.1%), A. phagocytophil

SUBMITTER: Sprong H 

PROVIDER: S-EPMC6401523 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature