Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay.

ABSTRACT:

Background

Enhancers are one of the most important classes of cis-regulatory elements (CREs) and play key roles in regulation of transcription in higher eukaryotes. Enhancers are difficult to identify because they lack positional constraints relative to their cognate genes. Excitingly, several recent studies showed that plant enhancers can be predicted based on their distinct features associated with open chromatin. However, experimental validation is necessary to confirm the predicted enhancer function.

Results

We developed a rapid enhancer validation system based on Nicotiana benthamiana. A set of 12 intergenic and intronic enhancers, identified in Arabidopsis thaliana, were cloned into a vector containing a minimal 35S promoter and a luciferase report

SUBMITTER: Lin Y 

PROVIDER: S-EPMC6402126 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature