Human Monoclonal Antibodies That Neutralize Pandemic GII.4 Noroviruses.

ABSTRACT:

Background & aims

Human noroviruses are responsible for approximately 200,000 deaths worldwide each year. In 2012, the GII.4 Sydney strain emerged and became the major circulating norovirus strain associated with human disease. Our understanding of the human norovirus-specific antibody response is limited because few human monoclonal antibodies (mAbs) to noroviruses have been described, and there are no functional assays to measure virus neutralization. We studied the antibody-mediated response to the genogroup (G) II.4 strain by isolating mAbs to GII.4 from infected patients and developing virus neutralization assays.

Methods

We used a robust human hybridoma technique to isolate mAbs from patients previously infected with norovirus and identified mAbs that blocked virus bin

SUBMITTER: Alvarado G 

PROVIDER: S-EPMC6402321 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature