Helios⁺ and Helios^- Treg subpopulations are phenotypically and functionally distinct and express dissimilar TCR repertoires.

ABSTRACT: The transcription factor Helios is expressed in a large subset of Foxp3⁺ Tregs. We previously proposed that Helios is a marker of thymic derived Treg (tTreg), while Helios^- Treg were induced from Foxp3^- T conventional (Tconv) cells in the periphery (pTreg). To compare the two Treg subpopulations, we generated Helios-GFP reporter mice and crossed them to Foxp3-RFP reporter mice. The Helios⁺ Treg population expressed a more activated phenotype, had a slightly higher suppressive capacity in vitro and expressed a more highly demethylated TSDR but were equivalent in their ability to suppress inflammatory bowel disease in vivo. However, Helios⁺ Treg more effectively inhibited the proliferation of activated, autoreactive splenocytes from scur

SUBMITTER: Thornton AM 

PROVIDER: S-EPMC6402968 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature