PvdF of pyoverdin biosynthesis is a structurally unique N¹⁰-formyltetrahydrofolate-dependent formyltransferase.

ABSTRACT: The hydroxyornithine transformylase from Pseudomonas aeruginosa is known by the gene name pvdF, and has been hypothesized to use N¹⁰-formyltetrahydrofolate (N¹⁰-fTHF) as a co-substrate formyl donor to convert N⁵-hydroxyornithine (OHOrn) to N⁵-formyl- N⁵-hydroxyornithine (fOHOrn). PvdF is in the biosynthetic pathway for pyoverdin biosynthesis, a siderophore generated under iron-limiting conditions that has been linked to virulence, quorum sensing and biofilm formation. The structure of PvdF was determined by X-ray crystallography to 2.3 Å, revealing a formyltransferase fold consistent with N¹⁰-formyltetrahydrofolate dependent enzymes, such as the glycinamide ribonucleotide transformylases, N-sugar transformylases and methiony

SUBMITTER: Kenjic N 

PROVIDER: S-EPMC6402982 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature