Proteomic changes in oocytes after in vitro maturation in lipotoxic conditions are different from those in cumulus cells.

ABSTRACT: Maternal lipolytic metabolic disorders result in a lipotoxic microenvironment in the ovarian follicular fluid (FF) which deteriorates oocyte quality. Although cellular stress response mechanisms are well defined in somatic cells, they remain largely unexplored in oocytes, which have distinct organelle structure and nuclear transcription patterns. Here we used shotgun proteomic analyses to study cellular responses of bovine oocytes and cumulus cells (CCs) after in vitro maturation under lipotoxic conditions; in the presence of pathophysiological palmitic acid (PA) concentration as a model. Differentially regulated proteins (DRPs) were mainly localized in the endoplasmic reticulum, mitochondria and nuclei of CCs and oocytes, however the DRPs and their direction of change were cell-type speci

SUBMITTER: Marei WFA 

PROVIDER: S-EPMC6403224 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature