A colorimetric strategy based on dynamic chemistry for direct detection of Trypanosomatid species.

ABSTRACT: Leishmaniasis and Chagas disease are endemic in many countries, and re-emerging in the developed countries. A rapid and accurate diagnosis is important for early treatment for reducing the duration of infection as well as for preventing further potential health complications. In this work, we have developed a novel colorimetric molecular assay that integrates nucleic acid analysis by dynamic chemistry (ChemNAT) with reverse dot-blot hybridization in an array format for a rapid and easy discrimination of Leishmania major and Trypanosoma cruzi. The assay consists of a singleplex PCR step that amplifies a highly homologous DNA sequence which encodes for the RNA component of the large ribosome subunit. The amplicons of the two different parasites differ between them by single nucleotide variat

SUBMITTER: Tabraue-Chavez M 

PROVIDER: S-EPMC6403333 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature