Comparative label-free lipidomic analysis of Mycobacterium tuberculosis during dormancy and reactivation.

ABSTRACT: Mycobacterium tuberculosis employs several strategies to combat and adapt to adverse conditions encountered inside the host. The non-replicative dormant state of the bacterium is linked to drug resistance and slower response to anti-tubercular therapy. It is known that alterations in lipid content allow dormant bacteria to acclimatize to cellular stress. Employing comparative lipidomic analysis we profiled the changes in lipid metabolism in M. tuberculosis using a modified Wayne's model of hypoxia-induced dormancy. Further we subjected the dormant bacteria to resuscitation, and analyzed their lipidomes until the lipid profile was similar to that of normoxially grown bacteria. An enhanced degradation of cell wall-associated and cytoplasmic lipids during dormancy, and their gradual restorati

SUBMITTER: Raghunandanan S 

PROVIDER: S-EPMC6403389 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature