Identification and Characterization of Novel Fc-Binding Heptapeptides from Experiments and Simulations.

ABSTRACT: Purification of biologically-derived therapeutics is a major cost contributor to the production of this rapidly growing class of pharmaceuticals. Monoclonal antibodies comprise a large percentage of these products, therefore new antibody purification tools are needed. Small peptides, as opposed to traditional antibody affinity ligands such as Protein A, may have advantages in stability and production costs. Multiple heptapeptides that demonstrate Fc binding behavior that have been identified from a combinatorial peptide library using M13 phage display are presented herein. Seven unique peptide sequences of diverse hydrophobicity and charge were identified. All seven peptides showed strong binding to the four major human IgG isotypes, human IgM, as well as binding to canine, rat, and mouse

SUBMITTER: Sun X 

PROVIDER: S-EPMC6404062 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature